Identification and expression of cuticular protein genes based on Locusta migratoria transcriptome

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Identification and expression of cuticular protein genes based on Locusta migratoria transcriptome

Many types of cuticular proteins are found in a single insect species, and their number and features are very diversified among insects. The cuticle matrix consists of many different proteins that confer the physical properties of the exoskeleton. However, the number and properties of cuticle proteins in Locusta migratoria remain unclear. In the present study, Illumina sequencing and de novo as...

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Effect of Ecdysterone on Food Intake of Locusta migratoria Hoppers

Insect growth and developm ent are controlled by hormones [1, 2], The horm ones were also shown to control food intake and utilization in som e insects [3-8]. Ecdysone is associated not only w ith m oult­ ing and m etam orphosis but also with processes preparatory to a moult, such as cessation o f feeding and the onset o f the w andering stage [9]. Recently evidence for a relationship between f...

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Plasma-desorption mass spectrometry as an aid in protein sequence determination. Application of the method on a cuticular protein from the migratory locust (Locusta migratoria).

The complete amino acid sequence of a structural protein, protein 8, isolated from the pharate cuticle of the locust Locusta migratoria was determined. Protein 8 contains 148 amino acid residues and has an Mr of 15,224. By the extensive use of information obtained by plasma-desorption mass spectrometry (p.d.m.s.) it was possible to reduce the need for conventional sequence determination and to ...

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Identification of Representative Genes of the Central Nervous System of the Locust, locusta migratoria manilensis by Deep Sequencing

The shortage of available genomic and transcriptomic data hampers the molecular study on the migratory locust, Locusta migratoria manilensis (L.) (Orthoptera: Acrididae) central nervous system (CNS). In this study, locust CNS RNA was sequenced by deep sequencing. 41,179 unigenes were obtained with an average length of 570 bp, and 5,519 unigenes were longer than 1,000 bp. Compared with an EST da...

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Large-Scale Transcriptome Analysis of Retroelements in the Migratory Locust, Locusta migratoria

BACKGROUND Retroelements can successfully colonize eukaryotic genome through RNA-mediated transposition, and are considered to be some of the major mediators of genome size. The migratory locust Locusta migratoria is an insect with a large genome size, and its genome is probably subject to the proliferation of retroelements. An analysis of deep-sequencing transcriptome data will elucidate the s...

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ژورنال

عنوان ژورنال: Scientific Reports

سال: 2017

ISSN: 2045-2322

DOI: 10.1038/srep45462